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A mortalin-like gene is crucial for planarian stem cell viability

Conte, Maria and Deri, Paolo and Isolani, Maria Emilia and Mannini, Linda and Batistoni, Renata (2009) A mortalin-like gene is crucial for planarian stem cell viability. Developmental Biology, 334 (1). pp. 109-118. ISSN 0012-1606

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    SUMMARY - In adult organisms, stem cells are crucial to homeostasis and regeneration of damaged tissues. In planarians, adult stem cells (neoblasts) are endowed with an extraordinary replicative potential that guarantees unlimited replacement of all differentiated cell types and extraordinary regenerative ability. The molecular mechanisms by which neoblasts combine long-term stability and constant proliferative activity, overcoming the impact of time, remain by far unknown. Here we investigate the role of Djmot, a planarian orthologue that encodes a peculiar member of the HSP70 family, named Mortalin, on the dynamics of stem cells of Dugesia japonica. Planarian stem cells and progenitors constitutively express Djmot. Transient Djmot expression in differentiated tissues is only observed after X-ray irradiation. DjmotRNA interference causes inability to regenerate and death of the animals, as a result of permanent growth arrest of stem cells. These results provide the first evidence that an hsp-related gene is essential for neoblast viability and suggest the possibility that high levels of Djmot serve to keep a p53-like protein signaling under control, thus allowing neoblasts to escape cell death programs. Further studies are needed to unravel the molecular pathways involved in these processes.

    Item Type: Article
    Uncontrolled Keywords: Planarian, Dugesia japonica, Stem cells, Neoblasts, Regeneration, Heat Shock Proteins, HSPs, Mortalin, RNAi
    Subjects: Area05 - Scienze biologiche > BIO/11 - Biologia molecolare
    Divisions: Dipartimenti (until 2012) > DIPARTIMENTO DI BIOLOGIA
    Depositing User: Prof. Renata Batistoni
    Date Deposited: 02 Feb 2010
    Last Modified: 20 Dec 2010 11:49
    URI: http://eprints.adm.unipi.it/id/eprint/643

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